Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (AdV-infected submandibular salivary gland)
Specification
AdV-infected submandibular salivary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX 0.2%
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C
Other product details
Dilution
1/800
Incubation time
1 hour(s) and 0 minute(s) · Diluent: blocking agent(10% Goat ser.,0.2% tritX)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit biotin + Streptavidin Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
PubMed ID
22942425: View
Additional Notes
PFA fixative 4% in PBS, 10' fixing time
Three-steps protocol: primary Ab (1:800), Secondary Goat anti-rabbit biotynilated (1:300), Tertiary Streptavidin Alexa488 (1:300)
All the Antibodies diluted in protein block: PBS 10% goat serum, 0.2% TritonX
Three-steps protocol: primary Ab (1:800), Secondary Goat anti-rabbit biotynilated (1:300), Tertiary Streptavidin Alexa488 (1:300)
All the Antibodies diluted in protein block: PBS 10% goat serum, 0.2% TritonX
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提交于 Oct 02 2012