Anti-Adenosine A1 Receptor抗体(ab82477)
Key features and details
- Rabbit polyclonal to Adenosine A1 Receptor
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-Adenosine A1 Receptor抗体
参阅全部 Adenosine A1 Receptor 一抗 -
描述
兔多克隆抗体to Adenosine A1 Receptor -
宿主
Rabbit -
特异性
Replenishment batches of ab82477 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab91076) -
阳性对照
- This antibody gave a positive signal in Rat Spinal Ganglion Neuronal cell lysate and Mouse EL4 Lymphoma whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82477于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11539 Mouse
- Entrez Gene: 29290 Rat
- SwissProt: Q60612 Mouse
- SwissProt: P25099 Rat
- Unigene: 298908 Mouse
- Unigene: 32078 Rat
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别名
- A1AR antibody
- A1R antibody
- AA1R antibody
see all
图片
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All lanes : Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/ml
Lane 1 : Rat Spinal Ganglion Neuronal cell lysate
Lane 2 :EL4 whole cell lysate (ab7183)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 118 kDa, 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
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IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab82477 被引用在 28 文献中.
- Guiol C et al. Adenosine Receptors Profile in Fibromuscular Dysplasia. Biomedicines 10:N/A (2022). PubMed: 36359350
- Lezmy J et al. Astrocyte Ca2+-evoked ATP release regulates myelinated axon excitability and conduction speed. Science 374:eabh2858 (2021). PubMed: 34648330
- Maille B et al. A2 Adenosine Receptor Subtypes Overproduction in Atria of Perioperative Atrial Fibrillation Patients Undergoing Cardiac Surgery: A Pilot Study. Front Cardiovasc Med 8:761164 (2021). PubMed: 34805317
- Chen ZH et al. Cordycepin Ameliorates Synaptic Dysfunction and Dendrite Morphology Damage of Hippocampal CA1 via A1R in Cerebral Ischemia. Front Cell Neurosci 15:783478 (2021). PubMed: 35002628
- Pan S et al. ADORA1 promotes nasopharyngeal carcinoma cell progression through regulation of PI3K/AKT/GSK-3ß/ß-catenin signaling. Life Sci 278:119581 (2021). PubMed: 33961854