Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP Human Cell Line)
Permeabilization
Yes - Ethanol
Specification
LNCaP Human Cell Line
Blocking step
Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Additional data
Additional Notes
Picture shows replicates of 4. Green channel is Activin Receptor IIB, Red channel represents DRAQ5 used to normalize for cell number.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
提交于 Mar 25 2015