Application
Western blot
Sample
Rat Tissue lysate - whole (liver, muscle, brain)
Loading amount
20 µg
Specification
liver, muscle, brain
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Concentration
1 µg/ml
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: DAKO cyto
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL plus
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 56 kDa Non-specific: 80, 112, 120 kDa
Positive control
Mucle
Negative control
Brain
Additional data
Additional Notes
Western Blot 10% PAGE probing with mouse monoclonal Activin (Ab89307) antibody. Lane 1, Embryonic day 18 (ED18) liver, lane 2: ED 18 muscles, Lane 3: ED18 cortex.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Sep 07 2010