Anti-Abhd5/CGI-58抗体(ab111984)
Key features and details
- Goat polyclonal to Abhd5/CGI-58
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Abhd5/CGI-58抗体
参阅全部 Abhd5/CGI-58 一抗 -
描述
山羊多克隆抗体to Abhd5/CGI-58 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 150-250 (internal sequence).
Database link: Q8WTS1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 39 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml.
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说明 |
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WB
Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 39 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. |
靶标
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功能
Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. -
组织特异性
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). -
疾病相关
Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma. -
序列相似性
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. -
发展阶段
Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level). -
结构域
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
细胞定位
Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. - Information by UniProt
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数据库链接
- Entrez Gene: 485570 Dog
- Entrez Gene: 51099 Human
- Entrez Gene: 67469 Mouse
- Entrez Gene: 497624 Pig
- Entrez Gene: 316122 Rat
- Omim: 604780 Human
- SwissProt: Q8WTS1 Human
- SwissProt: Q9DBL9 Mouse
see all -
别名
- 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
- ABHD5 antibody
- ABHD5_HUMAN antibody
see all
图片
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ab111984, at 3.75 µg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human kidney tissue by Immunohistochemistry.
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ab111984, at 3.75 µg/ml, staining Abhd5/CGI-58 in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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Anti-Abhd5/CGI-58 antibody (ab111984) at 0.2 µg/ml + NIH3T3 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab111984 被引用在 1 文献中.
- Gray NE et al. Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes. J Biol Chem 287:8444-56 (2012). WB ; Mouse . PubMed: 22267746