Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Colon
Blocking step
hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Picture MAX Polymer Detection Kit - Invitrogen Pol
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
No positive staining was found.
Abcam response
Thank you for having tested this antibody in an untested application (IHC-P). For your information, by participating in our Abtrial program you can use this product without financial risk by simply sharing your results for IHC-P. More information about this program can be found through this link: www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Vinicius Kannen Cardoso
Verified customer
提交于 Sep 15 2016