Anti-ATPB抗体[3D5] - Mitochondrial Marker (ab14730)
Key features and details
- Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-ATPB抗体[3D5] - Mitochondrial Marker
参阅全部 ATPB 一抗 -
描述
小鼠单克隆抗体[3D5] to ATPB - Mitochondrial Marker -
宿主
Mouse -
特异性
Human and Bovine complex V beta subunit (ATPB). -
经测试应用
适用于: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human, Caenorhabditis elegans
预测可用于: Goat, Cat, Dog, Pig, Common marmoset -
免疫原
Tissue, cells or virus corresponding to Human ATPB.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
3D5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14730于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (14) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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ICC/IF | (8) |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P | (10) |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
序列相似性
Belongs to the ATPase alpha/beta chains family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 327675 Cow
- Entrez Gene: 403669 Dog
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 100157156 Pig
- Entrez Gene: 171374 Rat
- GenBank: BC016512 Human
- Omim: 102910 Human
see all -
别名
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
see all
图片
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All lanes :
Lane 1 : HeLa
Lane 2 : HepG2
Lane 3 : Hu liver
Lane 4 : Ms liver
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDaMerged signal (red and green). Green - ab14730 observed at 52 kDa. Red - loading control, ab181602 observed at 37 kDa.
Samples were subjected to SDS-PAGE. ab14730 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1ug/mL and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab14730 staining ATPB in cultured human fibroblasts. Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor® 488-conjugated Goat anti-Mouse IgG1-specific secondary antibody (2 µg/ml)
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ab14730 (2µg/ml) staining ATPB in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining of epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ab14730 (blue) at 1µg/ml staining ATPB in HL-60 cells and analyzed by Flow cytometry. Red histogram represents equal quantity of isotype control.
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All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
Lane 1 : isolated mitochondria from human heart at 5 µg
Lane 2 : isolated mitochondria from bovine heart at 1 µg
Lane 3 : isolated mitochondria from rat heart at 10 µg
Lane 4 : isolated mitochondria from mouse heart at 10 µg
Predicted band size: 52 kDa
Observed band size: 52 kDa -
All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) at 0.8 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (229)
ab14730 被引用在 229 文献中.
- Pinson MR et al. Gag-like proteins: Novel mediators of prenatal alcohol exposure in neural development. Alcohol Clin Exp Res 46:556-569 (2022). PubMed: 35187673
- Marković A et al. Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat. Biomedicines 10:N/A (2022). PubMed: 35203486
- Mohás A et al. In Situ Analysis of mTORC1/C2 and Metabolism-Related Proteins in Pediatric Osteosarcoma. Pathol Oncol Res 28:1610231 (2022). PubMed: 35392503
- Li X et al. Ischemia-induced cleavage of OPA1 at S1 site aggravates mitochondrial fragmentation and reperfusion injury in neurons. Cell Death Dis 13:321 (2022). PubMed: 35395832
- Long M et al. DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion. EMBO J 41:e109390 (2022). PubMed: 35411952