Anti-ATP5F1抗体[9D1BC4] (ab117991)
Key features and details
- Mouse monoclonal [9D1BC4] to ATP5F1
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
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产品名称
Anti-ATP5F1抗体[9D1BC4]
参阅全部 ATP5F1 一抗 -
描述
小鼠单克隆抗体[9D1BC4] to ATP5F1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Human normal colon FFPE tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
9D1BC4 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117991于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 24.6 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
序列相似性
Belongs to the eukaryotic ATPase B chain family. -
细胞定位
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 515 Human
- Entrez Gene: 11950 Mouse
- Entrez Gene: 171375 Rat
- Omim: 603270 Human
- SwissProt: P24539 Human
- SwissProt: Q9CQQ7 Mouse
- SwissProt: P19511 Rat
- Unigene: 514870 Human
see all -
别名
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
see all
图片
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All lanes : Anti-ATP5F1 antibody [9D1BC4] (ab117991) at 1 µg/ml
Lane 1 : Human heart homogenate at 15 µg
Lane 2 : HepG2 lysate at 15 µg
Lane 3 : Human liver mitochondria at 7.5 µg
Lane 4 : Bovine heart mitochondria at 7.5 µg
Lane 5 : Rat liver mitochondria at 7.5 µg
Lane 6 : Mouse liver mitochondria at 7.5 µg
Predicted band size: 24.6 kDa
Note - Extra bands seen in lane 6 are secondary antibody mouse-on-mouse effects and not related to the primary antibody. -
ab117991 at 1 ug/ml with Hela cells in flow cytometry.
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Immunocytochemistry image of ab117991 (MS972) stained NIH3T3 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with urea/heat antigen retrieval method. The cells were incubated with ab117991 at 1 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the mitochondria. The four cells in the upper portion of the image show mitochondria in the elongated, reticular, arrangement, the three cells in the lower portion of the image show a punctuate mitochondrial organization and may be dividing/have recently divided.
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IHC image of ATP5F1 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117991, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (11)
ab117991 被引用在 11 文献中.
- Marković A et al. Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat. Biomedicines 10:N/A (2022). PubMed: 35203486
- Balderas E et al. Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment. Nat Commun 13:2769 (2022). PubMed: 35589699
- Jackson L et al. Activation of Gαq sequesters specific transcripts into Ago2 particles. Sci Rep 12:8758 (2022). PubMed: 35610292
- Hathaway QA et al. Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction. Part Fibre Toxicol 18:44 (2021). PubMed: 34911549
- Flax J et al. OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer's Disease Cytoplasmic Hybrids. J Alzheimers Dis 78:1743-1753 (2020). PubMed: 33285636