Anti-ATM (phospho S794)抗体(ab119799)
Key features and details
- Rabbit polyclonal to ATM (phospho S794)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ATM (phospho S794)抗体
参阅全部 ATM 一抗 -
描述
兔多克隆抗体to ATM (phospho S794) -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human ATM (phospho S794).
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阳性对照
- Jurkat, A431, HeLa, and Rat PC12 cells, treated with Calyculin A.
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常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119799于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 351 kDa.
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ICC |
1/200.
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说明 |
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WB
1/1000. Predicted molecular weight: 351 kDa. |
ICC
1/200. |
靶标
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功能
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. -
组织特异性
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes. -
疾病相关
Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.
Note=Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.
Note=Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).
Note=Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure. -
序列相似性
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 1 PI3K/PI4K domain. -
结构域
The FATC domain is required for interaction with KAT5. -
翻译后修饰
Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase.
Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. -
细胞定位
Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin. - Information by UniProt
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数据库链接
- Entrez Gene: 472 Human
- Entrez Gene: 11920 Mouse
- Omim: 607585 Human
- SwissProt: Q13315 Human
- SwissProt: Q62388 Mouse
- Unigene: 367437 Human
- Unigene: 5088 Mouse
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别名
- A-T mutated antibody
- A-T mutated homolog antibody
- AT mutated antibody
see all
图片
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ab119799 at 1/200 dilution staining ATM (phospho S794) in A431 cells treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to DyLight 594.
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Lanes 1-2 : Anti-ATM (phospho S794) antibody (ab119799) at 1/1000 dilution
Lanes 3-4 : Anti-
ATM (C-Terminal) at 1/1000 dilution
Lanes 5-6 : Anti-ATM (phospho S1981) at 1/1000 dilution
Lanes 1 & 3 & 5 : Human A431 cells treated with Calyculin A
(100 nM) for 30 min
Lanes 2 & 4 & 6 : Human A431 cells treated with Calyculin A
(100 nM) for 30 min, then treated with lambda phosphatase
Predicted band size: 351 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119799 被引用在 1 文献中.
- Zhang W et al. Nucleolar stress induces a senescence-like phenotype in smooth muscle cells and promotes development of vascular degeneration. Aging (Albany NY) 12:22174-22198 (2020). PubMed: 33146634