Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (HCT116 colon carcinoma)
Specification
HCT116 colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Li-CoR Donkey anti Rabbit Antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 800CW
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey Scanner
Bands
Specific: 350 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
lane 1: no drug
lane 2: etoposide 3 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 24 2014