Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial)
Loading amount
5000 cells
Specification
endothelial
Treatment
different shear stress exposure for 24 hours
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST (0.1%) with 1%BSA and azide
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Immobilon
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 45 kDa
Additional data
Additional Notes
Used with Ab47476 to give ratio of phospho v total
Cells were HUVEC (Human Umbilical Endothelial Cells)
Cells were cultured under different flow conditions, disturbed, normal and high shear stress.
Cells were HUVEC (Human Umbilical Endothelial Cells)
Cells were cultured under different flow conditions, disturbed, normal and high shear stress.
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提交于 Mar 05 2010