Anti-ASC1抗体(ab70627)
Key features and details
- Rabbit polyclonal to ASC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ASC1抗体
参阅全部 ASC1 一抗 -
描述
兔多克隆抗体to ASC1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide, corresponding to amino acids 225-275 of Human ASC1 (NP_057297.2)
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阳性对照
- HeLa whole cell lysate (ab150035) 293T whole cell lysate NIH3T3 whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70627于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function. -
细胞定位
Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation. - Information by UniProt
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数据库链接
- Entrez Gene: 9325 Human
- Entrez Gene: 56404 Mouse
- Omim: 604501 Human
- SwissProt: Q15650 Human
- SwissProt: Q9QXN3 Mouse
- Unigene: 500340 Human
- Unigene: 208379 Mouse
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别名
- Activating signal cointegrator 1 antibody
- ASC 1 antibody
- ASC-1 antibody
see all
图片
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ASC1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70627at 3 µg per reaction. Western blot was performed on the immunoprecipitate using ab70627 at 1 µg/mL.
Lane 1: ab70627 IP in HeLa whole cell lysate.
Lane 2: ab70627 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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All lanes : Anti-ASC1 antibody (ab70627) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 66 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ASC1 with ab70627 at 1/1000 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab70627 被引用在 9 文献中.
- Ouyang R et al. FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1. Mol Med Rep 25:N/A (2022). PubMed: 34850960
- Sun J et al. LncRNA H19 activates cell pyroptosis via the miR-22-3p/NLRP3 axis in pneumonia. Am J Transl Res 13:11384-11398 (2021). PubMed: 34786066
- Yang M et al. Colchicine Alleviates Cholesterol Crystal-Induced Endothelial Cell Pyroptosis through Activating AMPK/SIRT1 Pathway. Oxid Med Cell Longev 2020:9173530 (2020). PubMed: 32733639
- Liu H et al. Enhancer of zeste homolog 2 modulates oxidative stress-mediated pyroptosis in vitro and in a mouse kidney ischemia-reperfusion injury model. FASEB J 34:835-852 (2020). PubMed: 31914694
- Zhang J et al. Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis. Lipids Health Dis 19:39 (2020). PubMed: 32169116
- Li X et al. Klotho improves diabetic cardiomyopathy by suppressing the NLRP3 inflammasome pathway. Life Sci 234:116773 (2019). PubMed: 31422095
- Armstrong H et al. Nigericin Promotes NLRP3-Independent Bacterial Killing in Macrophages. Front Immunol 10:2296 (2019). PubMed: 31632394
- Davignon L et al. The transcription coactivator ASC-1 is a regulator of skeletal myogenesis, and its deficiency causes a novel form of congenital muscle disease. Hum Mol Genet 25:1559-73 (2016). PubMed: 27008887
- Thompson M et al. Paradoxical roles of serine racemase and D-serine in the G93A mSOD1 mouse model of amyotrophic lateral sclerosis. J Neurochem 120:598-610 (2012). PubMed: 22117694