重组Anti-ARTS1/ERAP1抗体[EPR6069] - BSA and Azide free (ab232466)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ARTS1/ERAP1抗体[EPR6069] - BSA and Azide free
参阅全部 ARTS1/ERAP1 一抗 -
描述
兔单克隆抗体[EPR6069] to ARTS1/ERAP1 - BSA and Azide free -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human bladder carcinoma tissue.
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常规说明
ab232466 is the carrier-free version of ab124669.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6069 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
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|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse and rat. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse and rat. |
靶标
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功能
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the peptidase M1 family. -
翻译后修饰
N-glycosylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 51752 Human
- Entrez Gene: 80898 Mouse
- Entrez Gene: 80897 Rat
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- SwissProt: Q9EQH2 Mouse
- SwissProt: Q9JJ22 Rat
- Unigene: 716426 Human
see all -
别名
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
图片
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Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab232466 被引用在 1 文献中.
- Seamon K et al. The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia. Front Immunol 11:396 (2020). PubMed: 32210971