Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Permeabilization
No
Blocking step
1% Donkey serum in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 488
Dilution
1/200
Additional data
Additional Notes
Green-ARF1, blue - nucleus (DAPI)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
提交于 Dec 05 2012