Application
Western blot
Sample
Human Cell lysate - whole cell (Hepato carcinoma HepG2 cells)
Gel Running Conditions
Reduced Denaturing (4 - 15%)
Loading amount
20 µg
Specification
Hepato carcinoma HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Positive control
HepG2 cells
Additional data
Additional Notes
Blots obtained with C-DiGit® Blot Scanner from LI-COR®
Lane 1: Molecular ladder
Lane 2 and 3: 20 µg of proteins
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 17 2016