Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (12)
Sample
Xenopus tropicalis Tissue lysate - whole (Brain)
Specification
Brain
Treatment
protease inhibitor cocktail treatment in RIPA lysis buffer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 48 kDa Non-specific: 40, 43 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
P19: mouse embryonic carcinoma cell line
mouse embryonic brain tissue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 24 2014