Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa WCE)
Loading amount
25 µg
Specification
HeLa WCE
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Blocker 1:1 in PBS + 0.1%Tween
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Odyssey® detection system
Exposure
1 second(s)
Bands
Specific: 35 kDa Non-specific: 30 kDa
Positive control
HeLa WCE & APE1 overexpression
Additional data
Additional Notes
Picture legend
1 - HeLa whole cell extract
2 - Overexpression of tagged APE1 in HeLa cells
Band at 30kDa could be APE1 degradation product, three weak bands above 40kDa correspond to ubiquitilated protein.
1 - HeLa whole cell extract
2 - Overexpression of tagged APE1 in HeLa cells
Band at 30kDa could be APE1 degradation product, three weak bands above 40kDa correspond to ubiquitilated protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Svetlana Khoronenkova
Verified customer
提交于 Aug 12 2010