Application
Western blot
Sample
Mouse Tissue lysate - whole (whole mouse heart)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
whole mouse heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% nonfat dry milk
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Alexa647 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Detection
Detection method
Fluorescence
Negative control
none
Exposure
3 second(s)
Positive control
none
Additional data
Additional Notes
WB image: MW markers, 50 ug mouse heart homogenate - not boiled (lane 1) and boiled (lane 2)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 16 2017