Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal West Dura
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~280 kDa Non-specific: 110 and 50 kDa kDa
Additional data
Additional Notes
This antibody readily detects a species of the predicted mobility in Human (MCF-7, lane 1) and Monkey lysate (Cos-7, lane 2). Little or no signal was observed in 3 rodent cell lines (lanes 3-5) suggesting that this antibody does not cross-react with rodent ANKRD11.
Lane order:
1, MCF-7 (Hu) 2, Cos-7 (AGMk) 3, CHO (Hm) 4, PC12 (Rat) 5, 3T3 (Ms)
Lane order:
1, MCF-7 (Hu) 2, Cos-7 (AGMk) 3, CHO (Hm) 4, PC12 (Rat) 5, 3T3 (Ms)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 13 2007