Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/100
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Dura
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: ~280 kDa Non-specific: 130, 90, 50 kDa
Additional data
Additional Notes
This antibody recognises a dominant species of the predicted mobility (~280 kDa) in both 293T and U2OS cell lysates. Several lower molecular weight cross-reactive bands are observed, some of which could be degradation products.
Lane order:
1, 293T 2, U2OS
Lane order:
1, 293T 2, U2OS
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 13 2007