Application
Western blot
Sample
Mouse Tissue lysate - whole (squeletal muscle, adipose tissus)
Gel Running Conditions
Reduced Denaturing (gradient gel 4-15%)
Loading amount
25 µg
Specification
squeletal muscle, adipose tissus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-tween 0.05 %
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
Pretty Good antibody. it worked very well in mouse squeletal muscles (gastrocnemius and soleus). We observed some extra bands in mouse adipose tissus. it Worked also in human hepg2 cells line.
Lane 1 : Mouse 1 squeletal muscle
Lane 2 : Mouse 2 squeletal muscle
Lane 3 : Mouse 3 squeletal muscle
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 22 2017