Anti-AMPK alpha 1抗体[2B7] (ab110036)
Key features and details
- Mouse monoclonal [2B7] to AMPK alpha 1
- Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-AMPK alpha 1抗体[2B7]
参阅全部 AMPK alpha 1 一抗 -
描述
小鼠单克隆抗体[2B7] to AMPK alpha 1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.
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阳性对照
- Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells WB: RAW 264.7, Mouse Liver, Neuro2A cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2B7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110036于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/1000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa. |
ICC/IF
1/200 - 1/1000. |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/1000. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5562 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: Q5EG47 Mouse
- SwissProt: P54645 Rat
- Unigene: 43322 Human
see all -
别名
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
图片
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All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution
Lane 1 : Wild-type RAW 264.7 cell lysate
Lane 2 : PRKAA1 knockout RAW 264.7 cell lysate
Lane 3 : Mouse Liver cell lysate
Lane 4 : Neuro2A cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDaFalse colour image of Western blot: Anti-AMPK alpha 1 antibody [2B7] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110036 was shown to bind specifically to AMPK alpha 1. A band was observed at 64 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in PRKAA1 knockout cell line ab280055 (knockout cell lysate ab280114). To generate this image, wild-type and PRKAA1 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : HeLa cell lysate.
Lane 3 : HepG2 cell lysate.
Lane 4 : MCF-7 cell lysate.
Lane 5 : Cos7 cell lysate.
Lane 6 : NIH3T3 cell lysate.
Lane 7 : K562 cell lysate.
Lane 8 : HEK293 cell lysate.
Lane 9 : PC-12 cell lysate.
Predicted band size: 64 kDa
Observed band size: 64 kDa -
The proposed dilution of ab110036 is 1/10000.
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ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.
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ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
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ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.
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ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab110036 被引用在 14 文献中.
- Fan H et al. Combined intake of blueberry juice and probiotics ameliorate mitochondrial dysfunction by activating SIRT1 in alcoholic fatty liver disease. Nutr Metab (Lond) 18:50 (2021). PubMed: 33971886
- Zhang Y et al. Neuromedin B receptor stimulation of Cav3.2 T-type Ca2+ channels in primary sensory neurons mediates peripheral pain hypersensitivity. Theranostics 11:9342-9357 (2021). PubMed: 34646374
- Li X et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol 11:73 (2020). PubMed: 32116781
- Yang Q et al. Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders. Front Cell Dev Biol 8:611354 (2020). PubMed: 33511118
- Xiong ZS et al. Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms. Mol Med Rep 20:887-894 (2019). PubMed: 31173255