Application
Western blot
Sample
Mouse Cell lysate - other (urine)
Loading amount
5 µg
Specification
urine
Treatment
CLB (NP40-based lysis buffer with protease inhibitors)
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0,1% Tween + 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti rabbit HRP, NA934, GE Healthcare
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 23 2011