Application
Western blot
Sample
Mouse Tissue lysate - whole (e7.5)
Loading amount
2 µg
Specification
e7.5
Gel Running Conditions
Reduced Denaturing (9% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/300
Detection
Detection method
ecl+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 16 kDa
Positive control
posterior embryo
Negative control
anterior embryo
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 31 2012