Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cancer cell line (A375))
Gel Running Conditions
Non-reduced Denaturing (4-20 % gel)
Loading amount
10 µg
Specification
Melanoma cancer cell line (A375)
Blocking step
Odyssey Block buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti Rabbit IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 57 kDa Non-specific: 40, 52, 60, 76, 102 kDa
Positive control
a-tub, seen as red band on the image, at correct size of 55kDa.
Additional data
Additional Notes
A few non specific bands, but clear to see that there is one very strong specific green band at around the correct size of 55kDa.
Abcam response
Thank you for sharing this data with us. Our lab suggest using 3-5% milk as the blocking buffer for ab52492, which should reduce the background you're seeing here.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Rebecca Atkinson-Dell
Verified customer
提交于 Aug 08 2017