Western blot abreview for Anti-AKT1 antibody [Y89]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma Initiating Cells (Primary cells))
Loading amount
30 µg
Specification
Glioblastoma Initiating Cells (Primary cells)
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/2500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Milk 1% in TBS Tween 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 100, 110 kDa

Additional data

Additional Notes
Ab32505 works fine at 1/5000 dilution
Username

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提交于 Nov 16 2011

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