Anti-AKR7A2抗体(ab97458)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to AKR7A2
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 84 - 357 of Human AKR7A2 (NP_003680).

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IF: HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab97458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/100 - 1/1000.

靶标

  • 功能
    Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
  • 组织特异性
    Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
  • 序列相似性
    Belongs to the aldo/keto reductase 2 family.
  • 细胞定位
    Golgi apparatus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AFAR antibody
    • AFAR1 antibody
    • AFB1 aldehyde reductase 1 antibody
    • AFB1 AR1 antibody
    • AFB1-AR 1 antibody
    • AFB1AR1 antibody
    • Aflatoxin aldehyde reductase antibody
    • Aflatoxin B1 aldehyde reductase member 2 antibody
    • Aflatoxin beta1 aldehyde reductase antibody
    • Aiar antibody
    • AKR7 antibody
    • Akr7a2 antibody
    • Aldo keto reductase family 7 antibody
    • Aldo keto reductase family 7 member A2 aflatoxin aldehyde reductase antibody
    • Aldo keto reductase family 7 member A2 antibody
    • Aldo keto reductase family 7, member A2 (aflatoxin aldehyde reductase) antibody
    • Aldoketoreductase 7 antibody
    • ARK72_HUMAN antibody
    • SSA reductase antibody
    • Succinic semialdehyde reductase antibody
    see all

图片

  • Anti-AKR7A2 antibody (ab97458) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
    10% SDS PAGE
  • ab97458, at a 1/200 dilution, staining AKR7A2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • Immunohistochemical analysis of PFA fixed, paraffin-embedded mouse fore brain tissue labeling AKR7A2 with a97458 at 1/500 dilution.

文献

ab97458 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (12% Tris/glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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