Application
Western blot
Sample
Human Tissue lysate - whole (endometrial cancer and adjacent control endometriu)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
endometrial cancer and adjacent control endometriu
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in T-TBS
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: peroxidase-conjugated IgG + IgM Jackson Immunorese
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Positive control
human placenta lysate, Ishikawa cell lysate
Additional data
Additional Notes
Band in placenta was very strong, therefore, we only 6 ug of placenta tissue lysate (5-fold less than other tissue and cell lysates).
PubMed ID
23146748: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Feb 12 2013