Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Loading amount
30 µg
Specification
Skeletal Muscle
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: T-PBS 1%
Secondary antibody
Name
Dilution
1/500
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 65, 57 kDa
Additional data
Additional Notes
Lane 1: Rat eskeletal muscle homogenate (control)
Lane 2: Rat eskeletal muscle homogenate (control)
Lane 3: Rat eskeletal muscle homogenate (apoptosis induced)
Lane 2: Rat eskeletal muscle homogenate (control)
Lane 3: Rat eskeletal muscle homogenate (apoptosis induced)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 15 2009