Application
ChIP
Sample
Human Cell lysate - other (Primary tonsillar B cells)
Specification
Primary tonsillar B cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Duration of cross-linking step: 4 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Taqman
Positive control
AID ChIP followed by qPCR analysis for known AID binding sites:
IgG switch region
IgM switch region
BIRC3 promoter
IgG switch region
IgM switch region
BIRC3 promoter
Negative control
AID ChIP followed by qPCR analysis for known sites where AID is known not to bind:
Neuro D1 gene
Neuro D1 gene
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
ChIP was performed on chromatin isolated from total primary human B cells before (0h) or after stimulation with IL-4 and anti-CD40Ab.
Chromatin was pre cleared before adding antibody 4.C for 4hrs
Antibody was pre-bound to beads 4.C for 4hrs and washed prior to adding pre-cleared chromatin.
Results were comparable to ab59361
100%ABR-T71V9
Chromatin was pre cleared before adding antibody 4.C for 4hrs
Antibody was pre-bound to beads 4.C for 4hrs and washed prior to adding pre-cleared chromatin.
Results were comparable to ab59361
100%ABR-T71V9
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. David Fear
Verified customer
提交于 Aug 13 2012