Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, U2OS, NIH-3T3, MEF)
Loading amount
20 µg
Specification
HeLa, U2OS, NIH-3T3, MEF
Gel Running Conditions
Reduced Denaturing (7.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
2 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk, PBS-Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal west dura
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: ~140 kDa Non-specific: 98, 64 and 50 (Stron kDa
Additional data
Additional Notes
Lane order (L-R): HeLa, U2OS, NIH-3T3, MEF
This antibody detects a product of the expected mobility (Swissprot: 138 kDa) in HeLa lysate. A strong band is observed at 52 kDa, which is consistent in size with an alternative AE2 transcript (variant e Aceview database)
This antibody detects a product of the expected mobility (Swissprot: 138 kDa) in HeLa lysate. A strong band is observed at 52 kDa, which is consistent in size with an alternative AE2 transcript (variant e Aceview database)
Abcam response
Inconclusive data on whether ab42687 works in Western Blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
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提交于 Jan 27 2009