Application
Western blot
Sample
Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Treatment
2uM Rosiglitazone for 24hrs
Specification
AML-12 cell(liver cell line)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5% milK
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 48 kDa Non-specific: 34, 90 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 30 2009