Application
Western blot
Sample
Human Cell lysate - whole cell (MDAMB231 breast cancer cells)
Loading amount
30 µg
Specification
MDAMB231 breast cancer cells
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.02% Tween-20 + 1% Non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: DAKO Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 35, 40 kDa
Positive control
Samples were stably transfected with ADAMTS15 or empty vector and the expression was validated with an anti-flag antibody against the tag on the protein. Bands of the correct size (105Kda) were seen with the flag antibody, only in those cells expressing ADAMTS-15.
Negative control
Cells transfected with empty vector
Additional data
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked under the experimental conditions you used. We hope that your data will be valuable to future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 05 2010