Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela Cells transiently overexpressing mouse ADAM17)
Specification
Hela Cells transiently overexpressing mouse ADAM17
Fixative
Methanol
Permeabilization
No
Other product details
Concentration
10 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
Cannot detect endogenous levels of ADAM17 in these cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 05 2008