Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sanger Diluent
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Cannot detect any staining above isotype control.
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested ab39161 for IHC in mouse tissue and your data suggest it may not be suitable for this species and application under the conditions in which you tested. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 25 2010