Application
Immunohistochemistry (Frozen sections)
Sample
Human Cell (skeletal muscle/frozen sections, human, rat and mo)
Permeabilization
Yes - 0,1 % Triton X-100 in PBS
Specification
skeletal muscle/frozen sections, human, rat and mo
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Histofix/Histolab/Sweden
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Mol
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
sample: human, rat and mouse skeletal muscle frozen section
dilution: 1:500 2 hours RT or overnight +4 (more diluted ones were not tested)
Note: cirka 16 % of population (human) is ACTN3 deficient (=no staining at all)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Satu Koskinen
Verified customer
提交于 Aug 01 2016