Anti-PGP9抗体(ab86222)
Key features and details
- Rabbit polyclonal to PGP9
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PGP9抗体 -
描述
兔多克隆抗体to PGP9 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human PGP9.
Database link: Q9NP78 -
阳性对照
- HeLa, 293T and mouse NIH3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
The membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications. -
细胞定位
Integral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum. -
数据库链接
- Entrez Gene: 23457 Human
- Entrez Gene: 56325 Mouse
- Entrez Gene: 63886 Rat
- Omim: 605453 Human
- SwissProt: Q9NP78 Human
- SwissProt: Q9JJ59 Mouse
- SwissProt: Q9QYJ4 Rat
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别名
- ABC transporter 9 protein antibody
- ABCB 9 antibody
- ATP binding cassette sub family B (MDR/TAP) member 9 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PGP9 with ab86222 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-PGP9 antibody (ab86222) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 10 seconds -
Detection of human PGP9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab86222 被引用在 1 文献中.
- Rodriguez-Navarro JA et al. Inhibitory effect of dietary lipids on chaperone-mediated autophagy. Proc Natl Acad Sci U S A 109:E705-14 (2012). WB ; Mouse . PubMed: 22331875