Anti-8 Hydroxyguanosine抗体[N45.1] (ab48508)


  • 产品名称Anti-8 Hydroxyguanosine抗体[N45.1]
    参阅全部 8 Hydroxyguanosine 一抗
  • 描述
    小鼠单克隆抗体[N45.1] to 8 Hydroxyguanosine
  • 特异性Nineteen analogues of 8-Hydroxy-2'-deoxyguanosine {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 免疫原

    Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin

  • 阳性对照
    • Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.



Our Abpromise guarantee covers the use of ab48508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

For mouse on mouse staining we recommend uisng MOM kit - ab127055


  • 相关性8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • 别名
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Anti-8 Hydroxyguanosine antibody [N45.1] 图像

  • De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-hydroxy-2′-deoxyguanosine (8OHdG) (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.

    Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.

  • ab48508 staining 8 Hydroxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.

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  • Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.
  • ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human spermatozoa by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using triton in 4% BSA for 15 minutes at room temperature, blocked with 4% BSA for 30 minutes at 20°C and then incubated using ab48508 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 350 conjugated goat anti-mouse monoclonal, used at a 1/250 dilution.

    Staining from left to right: 1. TUNEL 2. ab48508 3. DIC 4. Merge.

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Anti-8 Hydroxyguanosine antibody [N45.1] (ab48508)参考文献

This product has been referenced in:
  • Inoue S  et al. TAp73 is required for spermatogenesis and the maintenance of male fertility. Proc Natl Acad Sci U S A 111:1843-8 (2014). IHC-P . Read more (PubMed: 24449892) »
  • Chapmana AM  et al. IL-13 overexpression in mouse lungs triggers systemic genotoxicity in peripheral blood. Mutat Res 769:100-7 (2014). Read more (PubMed: 25400503) »

See all 11 Publications for this product

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I can recommend the antibody ab48508 against 8-hydroxy-2'-deoxyguanosine. This antibody has been tested in immunohistochemistry on paraffin embedded tissue sections (IHC-P).

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I am writing to you as I have been informed by our logistic group that ourAnti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1], AB48508, now ships with a concentration of 1.0mg/ml instead of 0.1mg/ml. As such the aliquot you receive will contain 50ug in a...

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Both these antibodies are designed to detect a specific DNA modification which is a species-independent reactivity. So yes, both antibodies are suitable for use in rat.

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I have heard back about ab48508.
The antibody ab48508 (anti 8-OHdG monoclonal antibody) is specific for
8-Hydroxy-2'-deoxyguanosine(8-OHdG). 8-Hydroxyguanosine (8OHG) demonstrate
minimal crossreactivity (less than 1%).
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