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Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin
This product was changed from ascites to tissue culture supernatant on 27th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab48508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
For mouse on mouse staining we recommend uisng MOM kit - ab127055.
De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-hydroxy-2′-deoxyguanosine (8OHdG) (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.
Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.
ab48508 staining 8 Hydroxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.
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