Anti-8 Hydroxyguanosine抗体(ab10802)

概述

  • 产品名称Anti-8 Hydroxyguanosine抗体
    参阅全部 8 Hydroxyguanosine 一抗
  • 描述
    山羊多克隆抗体to 8 Hydroxyguanosine
  • 特异性ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.
  • 经测试应用适用于: IHC-P, ELISA, ICC/IFmore details
  • 种属反应性
    Due to the conserved nature of the target reactivity would be predicted in wide range of species.
  • 免疫原

    8-Hydroxyguanosine - conjugate.

  • 阳性对照
    • Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Whole Serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200.

Perform enzymatic antigen retrieval with 10 µg/ml proteinase K for 40 minutes at 37°C.

ELISA 1/100000 - 1/250000.
ICC/IF Use at an assay dependent concentration. PubMed: 16678792

靶标

  • 相关性8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • 别名
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Anti-8 Hydroxyguanosine antibody 图像

  • IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.

Anti-8 Hydroxyguanosine antibody (ab10802)参考文献

This product has been referenced in:
  • Johansen AK  et al. The serotonin transporter promotes a pathological estrogen metabolic pathway in pulmonary hypertension via cytochrome P450 1B1. Pulm Circ 6:82-92 (2016). IHC-P ; Mouse . Read more (PubMed: 27162617) »
  • Zhang G  et al. The anti-oxidative role of Micro-vesicles derived from human Wharton-Jelly mesenchymal stromal cells through NOX2/gp91(phox) suppression in alleviating renal ischemia-reperfusion injury in rats. PLoS One 9:e92129 (2014). IHC-P ; Rat . Read more (PubMed: 24637475) »

See all 8 Publications for this product

Product Wall

This product has not been purified and is sold as a whole antiserum therefore we have not determined its concentration. As a rough guideline, in general whole antiserum has a concentration of 1-10 mg/ml.

The antibody will detect 8-OHdG in DNA and also RNA. RNase digestion recommended if you want to detect only 8-OHdG in DNA.

The paper at the following link used a different antibody, a mouse monoclonal antibody, but the procedure for staining ...

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The immunostain that used the antibody at 1/4000 used a biotin-conjugated secondary, so it is likely that this was followed with an avidin-biotin-HRP complex amplification. It is possible that 1/4000 was in fact effective, as antibody affinities vary a...

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Are you confident the secondary antibody and other reagents are effective? If so, I agree that it is best to try a different antibody at this point, which I will send free-of-charge.

Abcam has several others against 8-Hydroxyguanosine but as...

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Thank you for your enquiry.

I can confirm that ab10802 Anti-8 Hydroxyguanosine antibody is tested as suitable for indirect ELISA.

However, I am sorry to confirm that as far as we are aware, ab10802 has never been tested in flow cy...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (liver)
Specification liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 04 2013

Thank you for contacting us.

My Spanish speaking colleague is currently out of the office, unfortunately my Spanish is not good enough to write my reply in your language.

As stated on the product datasheets, both anti-8 Hydroxyguanosi...

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I appreciate the time taken to submit further information to us.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applicatio...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

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Wie am Telefon versprochen, sende ich Ihnen hier den Link zu der Publikation, von welcher das Protokoll als Vorbild genommen wurde:

http://www.ncbi.nlm.nih.gov/pubmed/11487050

Die Autoren der Publikation habeneinenmonoklonalen Antik...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"