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8-Hydroxyguanosine - conjugate.
Our Abpromise guarantee covers the use of ab10802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform enzymatic antigen retrieval with 10 µg/ml proteinase K for 40 minutes at 37°C.
|ELISA||1/100000 - 1/250000.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 16678792|
Immunohistochemical analysis of cat hepatic tissue, labeling 8 Hydroxyguanosine with ab10802. Sample fixed in paraformaldehyde. Heat mediated antigen retrieval with Tris-EDTA (pH9). Treated with ab10802 diluted 1/1500 in PBS plus casein for 1 hour and 30 minutes at 37°C. Secondary was ImmPress anti-goat HRP-conjugated polyclonal antibody.
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