Anti-8 Hydroxyguanosine抗体(ab10802)


  • 产品名称
    Anti-8 Hydroxyguanosine抗体
    参阅全部 8 Hydroxyguanosine 一抗
  • 描述
    山羊多克隆抗体to 8 Hydroxyguanosine
  • 宿主
  • 特异性
    ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    Due to the conserved nature of the target reactivity would be predicted in wide range of species.
  • 免疫原

    8-Hydroxyguanosine - conjugate.

  • 阳性对照
    • Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Whole Serum
  • 纯度
    Whole antiserum
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab10802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200.

Perform enzymatic antigen retrieval with 10 µg/ml proteinase K for 40 minutes at 37°C.

ELISA 1/100000 - 1/250000.


  • 相关性
    8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • 别名
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all


  • Immunohistochemical analysis of cat hepatic tissue, labeling 8 Hydroxyguanosine with ab10802. Sample fixed in paraformaldehyde. Heat mediated antigen retrieval with Tris-EDTA (pH9). Treated with ab10802 diluted 1/1500 in PBS plus casein for 1 hour and 30 minutes at 37°C. Secondary was ImmPress anti-goat HRP-conjugated polyclonal antibody.

    See Abreview

  • IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.


This product has been referenced in:
  • Jeong BY  et al. Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury. PLoS One 13:e0191034 (2018). Read more (PubMed: 29329317) »
  • Xia L  et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell 31:653-668.e7 (2017). Read more (PubMed: 28486105) »

See all 16 Publications for this product


Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

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Abcam has not validated the combination of species/application used in this Abreview.
IHC - Wholemount
Mouse Tissue (colon)

Dr. K Kulmira Nurgali

Verified customer

提交于 Nov 10 2017

This product has not been purified and is sold as a whole antiserum therefore we have not determined its concentration. As a rough guideline, in general whole antiserum has a concentration of 1-10 mg/ml.

The antibody will detect 8-OHdG in DNA and also RNA. RNase digestion recommended if you want to detect only 8-OHdG in DNA.

The paper at the following link used a different antibody, a mouse monoclonal antibody, but the procedure for staining ...

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The immunostain that used the antibody at 1/4000 used a biotin-conjugated secondary, so it is likely that this was followed with an avidin-biotin-HRP complex amplification. It is possible that 1/4000 was in fact effective, as antibody affinities vary a...

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Are you confident the secondary antibody and other reagents are effective? If so, I agree that it is best to try a different antibody at this point, which I will send free-of-charge.

Abcam has several others against 8-Hydroxyguanosine but as...

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Thank you for your enquiry.

I can confirm that ab10802 Anti-8 Hydroxyguanosine antibody is tested as suitable for indirect ELISA.

However, I am sorry to confirm that as far as we are aware, ab10802 has never been tested in flow cy...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Cat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 04 2013

Thank you for contacting us.

My Spanish speaking colleague is currently out of the office, unfortunately my Spanish is not good enough to write my reply in your language.

As stated on the product datasheets, both anti-8 Hydroxyguanosi...

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I appreciate the time taken to submit further information to us.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applicatio...

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