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Other Immunogen Type corresponding to 8 Hydroxyguanosine. 8-hydroxy-guanosine-BSA and –casein conjugates
Please see the protocol booklet link below for recommended IHC and ICC staining procedure
Our Abpromise guarantee covers the use of ab62623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 10 µg/ml.
Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum.
|ELISA||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/1000.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21824519|
Immunohistochemical analysis of murine brain tissue 24 hours after recirculation following ischemia. 8 Hydroxyguanosine was stained using ab62623 at 1/1000 dilution. An AlexaFluor®488-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling 8-Hydroxyguanosine with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.
ab62623 staining 8 Hydroxyguanosine in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.
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