RabMAb

Anti-70 kD Neurofilament Light抗体[EPR2459(2)] (ab108363)

概述

  • 产品名称Anti-70 kD Neurofilament Light抗体[EPR2459(2)]
    参阅全部 70 kD Neurofilament Light 一抗
  • 描述
    兔单克隆抗体[EPR2459(2)] to 70 kD Neurofilament Light
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 70 kD Neurofilament Light (internal sequence).

  • 阳性对照
    • C6, fetal brain and HeLa cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab108363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
IP 1/110.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    • 疾病相关Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
      Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
    • 序列相似性Belongs to the intermediate filament family.
    • 结构域The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
    • 翻译后修饰O-glycosylated.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 68 kDa neurofilament protein antibody
      • CMT1F antibody
      • CMT2E antibody
      • NEFL antibody
      • Neurofilament light polypeptide 68kDa antibody
      • Neurofilament light polypeptide antibody
      • Neurofilament protein light chain antibody
      • Neurofilament protein light polypeptide antibody
      • Neurofilament triplet L protein antibody
      • NF L antibody
      • NF-L antibody
      • NF68 antibody
      • NFL antibody
      • NFL_HUMAN antibody
      see all

    Anti-70 kD Neurofilament Light antibody [EPR2459(2)] 图像

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + C6 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • ab108363 (purified) at 1/110 immunoprecipitating 70 kDa Neurofilament Light in human fetal brain tissue lysate (Lane 1). For western blotting, a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/1000 dilution (unpurified)

      Lane 1 : C6 cell lysate
      Lane 2 : Human Fetal brain cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363)参考文献

    ab108363 has not yet been referenced specifically in any publications.

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