7-Chlorokynurenic acid (ab120024)

概述

  • 产品名称7-Chlorokynurenic acid
  • 描述
    NMDA receptor glycine site antagonist
  • 生物学描述Potent NMDA receptor glycine site antagonist. Water-soluble form available - please see 7-Chlorokynurenic acid sodium salt (ab120255).
  • 纯度> 99%

性能

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    应用

    Our Abpromise guarantee covers the use of ab120024 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    7-Chlorokynurenic acid 图像

    • ab12416 staining cGMP in SKNSH cells treated with 7-Chlorokynurenic acid (ab120024), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 7-Chlorokynurenic acid, as described in literature.
      The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120024 (7-Chlorokynurenic acid) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    7-Chlorokynurenic acid (ab120024)参考文献

    This product has been referenced in:
    • Moroni F  et al. Thiokynurenates: a new group of antagonists of the glycine modulatory site of the NMDA receptor. Eur J Pharmacol 199:227-32 (1991). Read more (PubMed: 1720099) »
    • Kleckner NW & Dingledine R Selectivity of quinoxalines and kynurenines as antagonists of the glycine site on N-methyl-D-aspartate receptors. Mol Pharmacol 36:430-6 (1989). Read more (PubMed: 2550776) »
    • Kemp JA  et al. 7-Chlorokynurenic acid is a selective antagonist at the glycine modulatory site of the N-methyl-D-aspartate receptor complex. Proc Natl Acad Sci U S A 85:6547-50 (1988). Read more (PubMed: 2842779) »

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