Potent NMDA receptor glycine site antagonist. Water-soluble form available - please see 7-Chlorokynurenic acid sodium salt (ab120255).
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in 1 eq. NaOH to 100 mM and in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab12416 staining cGMP in SKNSH cells treated with 7-Chlorokynurenic acid (ab120024), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 7-Chlorokynurenic acid, as described in literature. The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120024 (7-Chlorokynurenic acid) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Moroni F et al. Thiokynurenates: a new group of antagonists of the glycine modulatory site of the NMDA receptor. Eur J Pharmacol199:227-32 (1991).
Read more (PubMed: 1720099) »
Kleckner NW & Dingledine R Selectivity of quinoxalines and kynurenines as antagonists of the glycine site on N-methyl-D-aspartate receptors. Mol Pharmacol36:430-6 (1989).
Read more (PubMed: 2550776) »
Kemp JA et al. 7-Chlorokynurenic acid is a selective antagonist at the glycine modulatory site of the N-methyl-D-aspartate receptor complex. Proc Natl Acad Sci U S A85:6547-50 (1988).
Read more (PubMed: 2842779) »