10X His tag®多肽(ab14943)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
描述
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产品名称
10X His tag®多肽
参阅全部 10X His tag® 蛋白酶 -
纯度
> 90 % HPLC. -
无动物成分
No -
性质
Synthetic
相关产品
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Corresponding Antibody
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab14943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Blocking - Blocking peptide for Anti-6X His tag® antibody (ab14923)
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形式
Lyophilized -
补充说明
HIS-TAG® is a trademark of EMD Biosciences, Inc.- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C.
Information available upon request.
常规信息
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别名
- Dec-His tag
- Decahistidine tag
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细胞定位
Depends upon the localization of the parent protein tagged with Decahistidine.
图片
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To learn more about our protein and peptide range click here.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab14943 被引用在 4 文献中.
- Lovell JF et al. Interim analysis from a phase 2 randomized trial of EuCorVac-19: a recombinant protein SARS-CoV-2 RBD nanoliposome vaccine. BMC Med 20:462 (2022). PubMed: 36447243
- Siddiqui MF et al. Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32604729
- Chen J et al. Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection. PLoS Pathog 11:e1005053 (2015). PubMed: 26226614
- Rani N et al. FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis. Nat Commun 3:749 (2012). PubMed: 22434192