Anti-6X His tag®抗体(HRP) (ab3553)

概述

  • 产品名称
    Anti-6X His tag®抗体(HRP)
    参阅全部 6X His tag® 一抗
  • 描述
    鸡多克隆抗体to 6X His tag® (HRP)
  • 偶联物
    HRP
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 常规说明
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 骨髓瘤
    unknown
  • 同种型
    IgY
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. 1/1000.
ELISA Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.

靶标

  • 相关性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

实验方案

文献

This product has been referenced in:
  • Allan CM  et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). Read more (PubMed: 23270816) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (protein produced in mammalian expression system)
Loading amount
1 µg
Specification
protein produced in mammalian expression system
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2007

Thank you for your enquiry. Yes, this product will detect N-terminal HIS tags.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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