Anti-6X His tag®抗体(HRP) (ab1269)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/30000.
ELISA 1/10000 - 1/100000.
ICC 1/200 - 1/500.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (HRP) (ab1269)参考文献

This product has been referenced in:
  • Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . Read more (PubMed: 27378283) »
  • Seijsing J  et al. Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One 8:e81350 (2013). ELISA . Read more (PubMed: 24260574) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (BL21(DE3))
Loading amount 0.5 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 17 2010

I feel the best option would be for you to bind your mutant protein to the nickel plate. The ab1269 antibody can then be used to detect the his-tag on your mutant protein. It then leaves the HRP on the antibody free for detection. If you coat the p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Recombinant protein
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: PBS 10% FC
Type Direct
Username

Dr. Michael Aigner

Verified customer

提交于 Aug 15 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Sunil Verma

Verified customer

提交于 Aug 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"