Anti-6X His tag®抗体[HIS-1] (Alkaline Phosphatase) (ab49746)

概述

  • 产品名称Anti-6X His tag®抗体[HIS-1] (Alkaline Phosphatase)
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[HIS-1] to 6X His tag® (Alkaline Phosphatase)
  • 偶联物Alkaline Phosphatase
  • 特异性ab49746 recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • 经测试应用适用于: WB, Dot blot, ELISAmore details
  • 免疫原

    Recombinant poly(Histidine)-tagged fusion protein.

  • 阳性对照
    • Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

    The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • 纯化说明Immunoglobulin fraction purified from ascites fluid.
  • Primary antibody说明Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
  • 克隆单克隆
  • 克隆编号HIS-1
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 7 kDa.
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746)参考文献

This product has been referenced in:
  • Henche AL  et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). Read more (PubMed: 25102813) »
  • Hussack G  et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . Read more (PubMed: 21216961) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

The 6x His-tag should essentially be the same no matter which vector it is expressed by. The affinity of the antibody is more likely to be affected by the proximity of the His-tag to the recombinant protein (N...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal cell)
Loading amount 10 µg
Specification neuronal cell
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"