Anti-6X His tag®抗体[HIS-1] (ab49936)

概述

  • 产品名称Anti-6X His tag®抗体[HIS-1]
    参阅全部 6X His tag® 一抗
  • 描述
    小鼠单克隆抗体[HIS-1] to 6X His tag®
  • 经测试应用适用于: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
  • 免疫原

    Recombinant full length protein corresponding to 6X His tag®.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Proprietary Purification
  • 纯化说明Purified from Tissue culture supernatant.
  • 克隆单克隆
  • 克隆编号HIS-1
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
WB 1/3000.
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [HIS-1] (ab49936)参考文献

ab49936 has not yet been referenced specifically in any publications.

Product Wall

Using 50ul of diluted antibody per well is an adequate amount although this amount may be optimized in your experiments. As this product is an unpurified ascites fluid, I would recommend using a slightly higher concentration of this product closer to 1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"