Anti-6X His tag®抗体(FITC) (ab3554)

概述

  • 产品名称Anti-6X His tag®抗体(FITC)
    参阅全部 6X His tag® 一抗
  • 描述
    鸡多克隆抗体to 6X His tag® (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: WB, ELISA, IHC-Frmore details
  • 免疫原

    Synthetic peptide:

    HHHHHH

    conjugated to KLH.

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液PBS, 0.2% BSA, 0.1% sodium azide, pH7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 骨髓瘤unknown
  • 同种型IgY
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.

ELISA Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

IHC-Fr 1/100.

靶标

  • 相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) 图像

  • ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.

    See Abreview

Anti-6X His tag® antibody (FITC) (ab3554)参考文献

ab3554 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
Sample Rat Tissue sections (spinal cord tissue)
Specification spinal cord tissue
Permeabilization Yes - TritonX
Fixative Paraformaldehyde
Username

Dr. Hayk Petrosyan

Verified customer

提交于 Jun 23 2014

Thank you for your call today. Please see below for your discount code and for the details of the testing program. Let me know if you have any questions or if there is anything else that we can help you with.

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