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Our Abpromise guarantee covers the use of ab3554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by Western Blot.
|ELISA||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by ELISA.
ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.
ab3554 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"