Anti-6X His tag®抗体(FITC) (ab3554)

概述

  • 产品名称
    Anti-6X His tag®抗体(FITC)
    参阅全部 6X His tag® 一抗
  • 描述
    鸡多克隆抗体to 6X His tag® (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: WB, ELISA, IHC-Frmore details
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    PBS, 0.2% BSA, 0.1% sodium azide, pH7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 骨髓瘤
    unknown
  • 同种型
    IgY
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.

ELISA Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

IHC-Fr 1/100.

靶标

  • 相关性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) 图像

  • ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.

    See Abreview

Anti-6X His tag® antibody (FITC) (ab3554)参考文献

ab3554 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
Sample
Rat Tissue sections (spinal cord tissue)
Specification
spinal cord tissue
Permeabilization
Yes - TritonX
Fixative
Paraformaldehyde
Username

Dr. Hayk Petrosyan

Verified customer

提交于 Jun 23 2014

I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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