Anti-6X His tag®抗体(FITC) (ab1270)

概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    PBS with 0.2% BSA, 0.1% sodium azide
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/200 - 1/2000.

靶标

  • 相关性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献

ab1270 has not yet been referenced specifically in any publications.

客户评价及客户问答

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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